Regulatory

Part:BBa_K1968003:Design

Designed by: Alejandra   Group: iGEM16_Edinburgh_OG   (2016-10-11)


PglaA inducible promoter: glucoamylase gene promoter (PglaA) from Aspergillus niger


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 611
    Illegal SpeI site found at 280
    Illegal PstI site found at 147
    Illegal PstI site found at 489
    Illegal PstI site found at 605
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 611
    Illegal SpeI site found at 280
    Illegal PstI site found at 147
    Illegal PstI site found at 489
    Illegal PstI site found at 605
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 611
    Illegal SpeI site found at 280
    Illegal PstI site found at 147
    Illegal PstI site found at 489
    Illegal PstI site found at 605
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 611
    Illegal SpeI site found at 280
    Illegal PstI site found at 147
    Illegal PstI site found at 489
    Illegal PstI site found at 605
    Illegal NgoMIV site found at 525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 579


Design Notes

This part contains Phytobricks overhangs E and C, because this promoter includes the RBS and was the second promoter in our specific plasmid design.


Source

This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). After the part was cloned, its sequence was confirmed by Sanger Sequencing.

References